Alternative protein nanofibre scaffolds for multiple-type mucosal cells and tissue culture

This PhD project aims to engineer sustainable nanofiber scaffolds from alternative proteins for advanced mucosal tissue generation. Using electrospinning and surface modification, we will create biocompatible, adhesive scaffolds that support fibroblast growth and differentiation, forming a collagen-rich matrix. Co-culturing fibroblasts with mucin-producing epithelial cells will enable the development of a self-generating artificial mucosa, mimicking the architecture and mechanics of natural mucosal tissue. The study will investigate nutrient transport, metabolic profiles, and cellular resilience under environmental stresses, while characterising mechanical properties and biodegradability of the cultured tissue. Outcomes include a protein nanofiber–cell hybrid matrix, scalable fabrication protocols, and a prototype artificial mucosa for applications in biomedical research, wound healing, and mucus replacement therapies. This interdisciplinary project combines nanotechnology, cell biology, and bioprocessing, fostering collaboration between UoL and KCL to advance programmable biomaterials and next-generation tissue engineering.