Structure-function relationships of XNAzymes and XNA aptamers investigated by nanopore-enhanced native mass spectrometry

Engineered nucleic acids (xeno nucleic acids, XNAs) use chemical configurations not found in nature to extend their structural and functional scope, and are increasingly a source of novel nanostructures, -devices and drugs. In this project, you will study XNAs designed with specific enzymatic and high-affinity binding functionalities and elucidate structure-function relationships. You will develop and use structural mass spectrometry approaches including native MS together with nanopore technology and other biophysical characterization.
The project is led by Leeds and co-supervised by Frank Sobott (Leeds; structural MS), Alex Taylor (King’s College London; XNAs) and Paolo Actis (Leeds; nanopores). It provides a unique opportunity to work at the cutting edge of nanotechnology, bioengineering and biophysical/structural characterization, and to study engineered systems based on artificial genetic polymers with expanded physicochemical and pharmacodynamic properties.