Development of single molecule tools for introducing or extracting precise amounts of biological material into or from living cells. Development of functional DNA origami nanostructures for the characterizaiton of protein-protein interactions and single molecule biosensing
I use single-molecule imaging to map out protein distributions in 3D in fixed cells or cell-cell contacts to establish how separation between and clustering of proteins influence signalling processes. I also use single-molecule tracking to study how molecules bind to receptors. I have developed single-molecule flow cytometry for charactersing expression of low abundance protein targets…
My work focuses on label-free methodologies to tackle biomedical problems at the single-cell level. Specifically, Raman spectroscopy, both spontaneous Raman as well as SRS and CARS imaging, and microfluidics. I am the co-chair of the HYBIFA facility, able to do multiphoton imaging characterisation of biomedical samples using label-free vibrational imaging (SRS and CARS), second harmonic…
Understanding physico-chemical processes and spatial and temporal structural changes occurring during biomineralisation, and biomimetic engineering biology approaches to repair and replace lost hard tissues. Specifically, my expertise lies in a multimodal approach to drive forward our understanding of hard tissue structures at multiple length-scales and interactions at protein-mineral interfaces to enable translation of oral and…
My group has worked on a range of different systems and technologies from developing new small molecules, membrane protein scaffolds, biosensors and time-resolved approaches. We have an interest in engineering membrane proteins and lipid scaffolds and also cell free expression systems
Our lab investigates the molecular machinery driving cellular movement and force—focusing on the myosin superfamily in health and disease, and how mutations or drugs alter function. Using structural biology and protein engineering, we uncover dynamic mechanisms to inform precision therapies. Our work bridges biomolecular engineering and programmable microenvironments to manipulate force generation at the nanoscale.
We are developing polyvalent multifunctional nanoparticles (PMNs) to maximize the activity and accessibility of conjugated biomolecules and fully exploit multivalency to enhance targeting affinity and specificity. Our PMNs can be tailored for applications such as targeting antigen presenting cells to modulate their immune responses for potential immunotherapeutic treatment for allergy, cancer, and diabetes. They can…